SubsidieMeestersFast Electro-Optic Fluorescence Lifetime Microscopy (EOFLIM) for super-resolution microscopy and brain surgery.
The project develops a high-speed, sensitive EOFLIM detector for super-resolution and surgical applications, enhancing imaging capabilities in research and cancer diagnostics.
Projectdetails
Introduction
Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful method for monitoring physicochemical parameters of cells and tissues, as well as biomolecular interactions, with applications in science, medicine, and industry. However, its use is currently limited due to technological constraints regarding frame rate and sensitivity.
Development of EOFLIM Detector
We have developed a FLIM detector that enables highly sensitive, high-speed FLIM. It is based on the use of electro-optical elements as temporal gating units.
Features of EOFLIM
Electro-optical FLIM (EOFLIM) enables:
- Frame rates beyond 100 Hz for megapixel images
- Single-molecule sensitivity
- High-resolution imaging (numerical aperture > 1.3)
Key Applications
We will demonstrate the innovation potential of our detector in two key applications:
-
Super-resolution FLIM
This will be done in collaboration with Prof. Huser (Univ. of Bielefeld). We will apply our EOFLIM detector to super-resolution microscopy at high frame rates in live cells and tissues. -
Surgical FLIM
This will be done in collaboration with Prof. Unterhuber (Medical Univ. of Vienna) and will aim at diagnosing cancerous tissue in brain biopsies.
Conclusion
Super-resolution FLIM and Surgical FLIM will demonstrate the wide applicability of EOFLIM in research and medicine.
Financiële details & Tijdlijn
Financiële details
| Subsidiebedrag | € 150.000 |
| Totale projectbegroting | € 150.000 |
Tijdlijn
| Startdatum | 1-7-2022 |
| Einddatum | 31-12-2023 |
| Subsidiejaar | 2022 |
Partners & Locaties
Projectpartners
- UNIVERSITAT WIENpenvoerder
Land(en)
Geen landeninformatie beschikbaar
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Optical Microscope for Imaging High-Speed Precision Surface Processes
SURFLIGHT aims to revolutionize optical microscopy with a high-resolution, real-time microscope for monitoring ultrafast surface processes, enhancing catalysis R&D and reducing costs across various industries.
A light-efficient microscope for fast volumetric imaging of photon starved samples
LowLiteScope aims to revolutionize bioluminescence microscopy by using AI-driven light field techniques for high-resolution 3D imaging of biological samples, enhancing research capabilities in life sciences.
Super-resolution Field-Resolved Stimulated Raman Microscopy
This project aims to develop a super-resolution, label-free Raman microscope using femtosecond laser technology to non-invasively visualize subcellular structures with unprecedented sensitivity and resolution.
Time-based single molecule nanolocalization for live cell imaging
The project aims to develop a novel live-cell nanoscopy technique that enables high-speed, high-resolution imaging of biological processes at the nanoscale without compromising depth or volume.
Next-gen fluorescence imaging for research and theranostics
The project aims to develop the TriScanner, a novel fluorescence microscope that enhances imaging speed, resolution, and sensitivity for multicellular systems in research and clinical applications.
Vergelijkbare projecten uit andere regelingen
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Breaking the Resolution Limit in Two-Photon Microscopy Using Negative Photochromism
This project aims to develop a novel multiphoton microscopy technique that achieves four-photon-like spatial resolution using two-photon absorption, enhancing biomedical imaging capabilities.
Development of an In-Vivo Brillouin Microscope (with application to Protein Aggregation-based Pathologies)
This project aims to enhance Brillouin Microscopy for real-time, non-destructive assessment of viscoelastic properties in living cells, addressing key biomedical challenges.