Biomimetic Sensorized Barriers-on-a-Chip: Unveiling a new Generation of Market-Ready Investigation Tools

Introduction

The lack of reliable in vitro pre-clinical models represents a critical aspect unanimously recognized by the scientific community as detrimental in the pathway of bringing new medications from the laboratory to the bedside. Addressing this issue would provide immense benefits to drug discovery and development processes by ethically limiting animal testing and drastically reducing costly failures in clinical trials.

Current Challenges

To date, most in vitro models fail at recapitulating the physiological microenvironment, and this often results in misleading data withdrawal.

Project Overview

Starting from my prototype of a real-scale, dynamic, and biomimetic blood-brain barrier model, in this project I aim at the validation of my system. This will follow an upgrade step consisting of embedding sensing features in the platform, thus allowing a real-time evaluation of barrier formation and integrity maintenance.

Innovation

Characterized by microcapillary size/fenestrations and fluid flows similar to the in vivo physiological barrier, my tool will represent a drastic innovation over other well-established models in the literature and available on the market. It will allow a reliable reproduction of the physiological environment and an accurate estimation of the amount of drug and/or nanomaterial concentration delivered through the barrier.

Validation Process

Validation will be performed in relevant conditions by implementing stroke and brain cancer models.

Fabrication Technique

All artificial components will be fabricated through two-photon polymerization (2pp), a disruptive mesoscale lithography technique that allows the fast fabrication of low-cost structures with resolution up to the nanometer scale, as well as great levels of scaffold reproducibility and accuracy.

Applications

The proposed platform can be easily adopted in research laboratories and pharmaceutical industries as an advanced pre-clinical model. The primary biomedical applications of this model consist of reliable screenings of drugs against pathologies of the central nervous system.