A new technology to probe molecular interaction in cells at high throughput
The DiffusOMICS project aims to develop a high-throughput fluorescence-based method to map molecular interactions and detect protein aggregates in neurons for improved drug screening.
Projectdetails
Introduction
Protein oligomers and aggregates are pillars of many cellular processes and can be associated with a number of life-threatening neurodegenerative diseases. These processes are often context-dependent but are hard to detect in situ with available technologies. The ideal instrument can target specific proteins of medical interest in their physiological contexts, resolve nano-distances (1-100 nm), and capture dynamics of association and dissociation (ns-ms).
Project Aim
The DiffusOMICS project aims to fill this methodological gap by prototyping a high-throughput fluorescence-based approach able to measure the rotational diffusion properties of molecules in vivo and in situ.
Concept Overview
The concept is based on our recent publication, where we utilize intelligent fluorescent probes together with polarized light to investigate the rotational dynamics of large molecular complexes.
Sensitivity of Rotational Diffusion
Rotational diffusion is highly sensitive to changes in mass, making the technique ideal to detect the presence of aggregates and report on molecular size and local viscosity.
Measurement Capabilities
Due to the nature of the signal, the measurements can be done in cells and tissues at high throughput, making the technology appealing for industrial screening applications.
Project Goals
The goal of the project is to establish both the technological foundation and the collaborations needed to develop a prototype instrument and to record the very first high-throughput molecular interaction maps in neurons.
Potential Impact
DiffusOMICS possesses all the essential qualities to become a game-changing technology for drug screening due to its sensitivity, specificity, and speed, making it suitable for automated recordings.
Mapping Capabilities
DiffusOMICS maps will carry information on the whole population of aggregates localized in different neuronal compartments and can track changes in aggregation responses to drug perturbations and mutagenesis in neuronal proteins.
Conclusion
The outcome will be key to engaging industrial strategic partners and it paves the way for a new paradigm of drug screening in situ.
Financiële details & Tijdlijn
Financiële details
Subsidiebedrag | € 150.000 |
Totale projectbegroting | € 150.000 |
Tijdlijn
Startdatum | 1-4-2024 |
Einddatum | 30-9-2025 |
Subsidiejaar | 2024 |
Partners & Locaties
Projectpartners
- KUNGLIGA TEKNISKA HOEGSKOLANpenvoerder
Land(en)
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measuriNg nEURal dynamics with label-free OpticaL multI-DomAin Recordings
This project aims to innovate label-free optical methods for monitoring neural dynamics in the brain, enhancing understanding and treatment of brain diseases without exogenous reporters.
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This project aims to investigate the biophysical mechanisms of protein aggregation in Huntington's Disease using advanced imaging techniques to enhance understanding of neurodegenerative processes.
Single-Molecule Acousto-Photonic Nanofluidics
SIMPHONICS aims to develop a high-throughput, non-invasive platform for protein fingerprinting by integrating nanopore technology with acoustic manipulation and fluorescence detection.
Global Amyloid Mapping: Solving Amyloid Nucleation by Deep Mutagenesis
This project aims to map mutations affecting amyloid nucleation, model transition states, and identify stress-responsive sequences to enhance understanding and treatment of amyloid-related diseases.
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