Next-generation engineering of gas-fermenting cell factories through large-scale systems-level maps

Introduction

Gas fermentation provides a unique opportunity for the circular bioeconomy by enabling carbon recycling from gaseous waste feedstocks into value-added bioproducts using microbes. Acetogens are ideal biocatalysts for gas fermentation as they use gas (CO and/or CO2+H2) as their sole carbon and energy source, evidenced by the commercialization of the waste gas-to-ethanol conversion process.

Current Understanding

Despite recent efforts, our understanding of genotype-phenotype relationships in acetogens is still minimal. This, however, is required for rational metabolic engineering and expanding the product spectrum of acetogens to unlock their full potential.

Project Goals

GENESYS aims to engineer superior gas-fermenting cell factories through:

1. Revolutionizing systems-level understanding of acetogens.
2. Pioneering a new workflow for making large-scale arrayed CRISPR-engineered microbial strain libraries.

Methodology

Gene Identification

First, essential genes for autotrophy of the model acetogen Clostridium autoethanogenum will be determined using a genome-wide CRISPRi screen.

Strain Creation

We then create approximately 750 single-gene knock-down strains of essential genes, transcription factors, and proteome dark matter using a novel pooled-to-arrayed workflow combining synthetic biology, automation, and DNA sequencing.

Phenotype Mapping

Next, we will generate systems-level phenotype maps for up to approximately 750 strains through high-throughput gas fermentation and omics analyses.

Data Integration

Big data will be translated into a systems-level acetogen knowledgebase, A-BASE, through integrated bioinformatics and machine learning, metabolic modeling, and data engineering.

Final Application

Finally, we apply A-BASE to engineer acetogen cell factories from the unexplored territories of essential genes, transcription factors, and proteome dark matter for improving production of ethanol and acetone.

Conclusion

This project will create superior gas-fermenting cell factories, advance fundamental understanding of acetogen metabolism, and pioneer a new workflow for creating large-scale arrayed engineered microbial strain libraries.