Living Therapeutic and Regenerative Materials with Specialised Advanced Layers

Introduction

Skin is a living interface with layers of specialised functions (sensing, regeneration, protection). The level of complexity in skins is currently unreached in engineered living materials (ELMs). Here we will create skin-inspired ELMs with layers with living cells, specialised properties and functions.

Living Skins Development

Our living skins are based on a grown matrix hosting engineered multicellular consortia that build and functionalise different layers. Spatiotemporal patterning is realised by genetic control and the physicochemical properties of cells and biomolecules. Our aim is to generate platform technologies to advance biological ELMs and make two proof-of-concept engineered living skins with different applications:

Living Therapeutic Skin (LTS)

1. We will fabricate a self-encapsulated Living Therapeutic Skin (LTS) made of a bacterial cellulose hydrogel matrix with sense-and-respond cells.
2. LTS will have:
   • An interactive layer with sensing functions
   • A core layer for responsive living cells
   • A barrier layer for biocontainment and hydration

An example LTS will be designed to sense pathogenic skin bacteria in eczema and release biosynthesised therapeutic molecules.

Living Regenerative Skin (LRS)

1. A dry and tough Living Regenerative Skin (LRS) consisting of biomineralized biopolymers hosting bacterial spores will be fabricated as an alternative to traditional inert materials (ceramics, plastics) in protective garments.
2. The LRS biomineralized core will arrange in microscale layers, like tough biominerals in nature (nacre, bone, dentin).
3. LRS will be encapsulated in an activator shell, engineered to:
   • Prevent water penetration
   • Memorize local mechanical experience, giving local self-reinforcement of mechanically stressed regions

This provides a unique property compared to current materials and other ELMs.

User Involvement Strategy

As a powerful strategy to accelerate the adoption of our living materials in society, we will systematically involve the potential end-users and designers in our research for the materials and product development to happen in synergy.