A novel approach for studying biological proton transfer: Protein incorporation of noncanonical amino acids carrying a light-triggered proton donor and proton acceptor
This project aims to develop a novel method for directly measuring proton transfer reactions in proteins using light-activated noncanonical amino acids, enhancing our understanding of biological processes.
Projectdetails
Introduction
Proton transfer (PT) reactions within proteins are fundamental in biological systems, such as within ATP production. To date, there are no direct means to measure specific PT pathways within proteins, and most research is based on following the end-product of the PT reaction across a natural proton pathway.
Objective
Here, our goal is to develop a novel way to directly measure PT within proteins. Our new approach is based on placing proton donors and acceptors in specific places within proteins, in which the PT will be initiated only after light excitation of the system.
Methodology
To do so, we introduce here two noncanonical amino acids (ncAA) we developed, with a photoacid and a photobase as their residues that serve as the proton donor and acceptor, respectively. Our hypothesis is that the strong light-triggered driving force of PT in the excited state (of ~11 pKa units) will initiate PT along the pathway from donor to acceptor.
First Objective
In the first objective, we will use our new ncAA with solid-phase peptide synthesis to design several peptide systems that will allow us to decipher the role of:
- Specific amino acids
- Peptide structure
- The role of water in PT across the peptide using various ultrafast spectroscopy.
Second Objective
In the second objective, we aim to design a mutually orthogonal system for the insertion of our two ncAA into a single protein.
Third Objective
In our third objective, we plan to use our new experimental system for answering a unique set of questions in the field of biological PT that could not have been answered before, focusing on the systems of:
- ATP synthase transmembrane complex
- The soluble carbonic anhydrase enzyme.
Conclusion
Our new approach is groundbreaking in the way we study and understand PT in biology and will enable researchers to gain completely new capabilities resulting in fascinating new discoveries. Moreover, our new system can be translated into other fields in biology that require the local change in proton concentration within proteins.
Financiële details & Tijdlijn
Financiële details
Subsidiebedrag | € 2.190.000 |
Totale projectbegroting | € 2.190.000 |
Tijdlijn
Startdatum | 1-10-2024 |
Einddatum | 30-9-2029 |
Subsidiejaar | 2024 |
Partners & Locaties
Projectpartners
- TECHNION - ISRAEL INSTITUTE OF TECHNOLOGYpenvoerder
Land(en)
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