Yeast cell factory for mRNA bioproduction

Introduction

Messenger RNA (mRNA) vaccines have become a game-changer in the fight against COVID-19, providing an exit strategy to the global crisis of our age. mRNA is now considered a promising biopharmaceutical for a vast array of medical applications.

Current Challenges

Currently, in vitro transcription (IVT) is the only available technology for mRNA production; however, it relies on a complex supply chain and costly purification process. Reliable large-scale IVT production is challenging, and high production costs are an issue for low-income countries. This will be even more critical for clinical mRNA applications requiring high doses.

Yscript's Innovation

Yscript's groundbreaking nature lies in the generation of a specific mRNA bioproduction process in yeast, integrating innovative extraction and purification processes. This represents a complete shift of paradigm compared to IVT production and a revolutionary new use of yeast.

Advantages of Yeast

Although yeast possesses a similar mature mRNA structure as humans and is recognized as safe for pharmaceutical production, mRNA bioproduction and isolation have never been attempted.

Technological Improvements

Yscript will provide groundbreaking technological improvements by:

1. Decreasing the mRNA production supply chain.
2. Increasing the purity yield of long mRNA by avoiding premature termination occurring during IVT.
3. Guaranteeing mRNA stability through integrated extraction-purification processes.

This will ensure the generation of high-quality mRNA therapeutics, while streamlining production and reducing costs.

Expertise and Impact

Yscript brings together world-class expertise in:

• Molecular biology
• Biophysics
• Biotechnology
• Chemistry
• Chemical engineering
• mRNA therapeutics
• Yeast biology
• Bioproduction and purification processes

The project will significantly advance the current state of the art of mRNA manufacturing. Yscript will foster citizens' well-being and health, while decreasing economic and social disparities associated with biopharmaceutical-based therapies, in which mRNA is nowadays a key element.